Acquiring and processing a ¹H NMR spectrum on the Bruker ARX-300

Things in bold should be typed on the command line

Things underlined are buttons on the left-hand side panel of XwinNMR.

Things italicized are buttons on the BSMS keyboard.

1. Insert sample	If the lock is turned on (LED lit or blinking) , press Lock On/Off to turn lock off If the spinner is on (LED lit or blinking), press Spin On/Off to stop the spinner Press Lift On/Off to eject the standard Put your sample in the spinner, position it with the depth gauge, and place it in the bore Press Lift On/Off to insert your sample Press Spin On/Off to turn on the spinner
2. Set up acquisition parameters	edc Enter a NAME for the experiment. Don’t use special characters (\$@#”^/ etc.). Use _ or – instead of white space. Set the EXPNO (experiment number) Click SAVE rpar 1h_std all reads standard 1H parameters
3. Lock	If the lock display window is not open: lockdisp lopoi and select your solvent from the list Press Lock On/Off on BSMS Press Lock Gain and set it at 120 ± 5 Press Lock Power and adjust value to bring the lock level to the upper third of the lock display window
4. Shim	Press Lock Phase and adjust for maximum lock response Press Z and adjust for maximum lock response Press Z2 and adjust for maximum lock response Iterate between Z and Z2 until the signal reaches maximum Press Standby
5. Acquire FID	rga automatic receiver gain adjustment ns to change number of scans, if desired zg zero file and go
6. Window function & Fourier transform	Wait for acquisition to finish efp apply standard window function and FT
7. Phase	Click the |<>| button to display the whole spectrum Click the PHASE button to the left of the spectrum window (If you don’t see a PHASE button click RETURN first) Click the *2 button until details of the baseline are clear Click BIGGEST Click & Hold PH0 Drag the mouse up (or down) to adjust the phase of the tallest peak Click & Hold PH1 Drag the mouse up (or down) to adjust the phase of all other peaks Click RETURN à Save and Return
8. Reference	Zoom in on the reference peak (Left click, middle click, middle click, left click) Click CALIBRATE Set the cursor on top of the reference peak Middle click and enter the reference value
9. Peak Pick	Click UTILITIES Click MI and click OK if you get a pop-up window Move the mouse up or down to position the blue line. Anything below the line will not be peak picked Click RETURN
10. Baseline correct	abs Don’t use abs after integration!
11. Integrate	Click the INTEGRATE button to the left of the spectral window Click inside the spectral display Middle click to start an integral region Middle click to close an integral region Move around the spectrum using the < and > buttons Zoom in and out using the <> and >< buttons Repeat until all peaks are integrated Click RETURN à Save and Return
12. Plot	Click UTILITIES Click CY and set the blue line slightly above the tallest peak you want plotted. Left click and enter 10 as the height of the peak. Click RETURN Click DP1 Enter values for upper and lower plot limits plot To print expansion plots: Zoom in on the desired region Adjust CY as above, if desired Click the PLOT button
13. Eject sample	Press Lock On/Off to turn lock off Press Spin On/Off to stop the spinner Press Lift On/Off to eject your sample Put the standard in the spinner and place it in the bore of the magnet Press Lift On/Off to insert the standard