GC-MS update (04/15/21)

The Cornell Biotechnology Resource Center's Proteomics and Metabolomics Facility has agreed to let us run samples on their GC-MS system while we work to get a functioning turbopump for ours.

It's a modern, high-resolution, accurate-mass spectrometer, that is, frankly, much better than the GCMate in almost every respect. It is however not set up for open access, so Tony and I will have to take your samples there and run them for you.

Here is what to do if you want a GC-MS analysis:

1. Get autosampler vials from the AV400 prep table in B-73

2. Prepare your sample

   • Use a low-boiling, apolar solvent of high purity

   • Avoid diethyl ether and THF if you can as your bulk solvent. Small amounts are OK.

   • Chlorinated solvents are fine for MS (Don't use them for GC-FID)

   • Concentration should be less than 1 mM (~100 µg/ml). Calculate and measure! Don't guesstimate

   • Total sample volume should be between 0.5 and 2 ml. If you want to make smaller samples, ask us for a microvolume insert.

3. Label your sample with NetID and sample identification

4. Prepare an information sheet

5. Put your sample(s) and the information sheet(s) in a Ziploc bag and put the bag in the carboard box on the AV400 prep table in B-73

Questions, concerns, comments? Contact Ivan Keresztes, B-54, 255 0709, ik54@cornell.edu